癌-睾丸抗原CT63在慢性髓系白血病中的作用及其机制

孔汝心, 周亚群, 魏婷宜, 雷鸣

Function and mechanism of cancer-testis antigen CT63 in chronic myeloid leukemia

KONG Ruxin, ZHOU Yaqun, WEI Tingyi, LEI Ming

图5 CT63对细胞代谢的调控作用 Note： A. Activity of mitochondria complex Ⅳ in shCtrl and shCT63 K562 cells. B. Immunofluorescence staining of mitochondrial markers in tumors. PD—pyruvate dehydrogenase C. Relative fluorescence density of immunofluorescence staining in B. D. Western blotting analysis of mitochondrial markers in shCtrl and shCT63 K562 cells with PMA treatment. E. Relative average fluorescence density of Western blotting analysis. F. Immunofluorescence staining and subcellular localization of COX Ⅳ, G. Relative mtDNA level in shCtrl and shCT63 K562 cells. ①P=0.006, ②P=0.000, ③P=0.021, ④P=0.007, ⑤P=0.001, ⑥P=0.000, ⑦P=0.000, ⑧P=0.001, ⑨P=0.000, ⑩P=0.035, compared with the shCtrl group.

Fig 5 Regulation of CT63 on cell metabolism