双同源盒诱导小鼠胚胎干细胞向胚外内胚层分化的机制研究

洪磊, 郭传亮, 蔡勤, 李婉睿, 曾溢滔, 薛燕, 曾凡一

Mechanism of DUX-induced differentiation of mESC into extraembryonic endoderm

HONG Lei, GUO Chuanliang, CAI Qin, LI Wanrui, ZENG Yitao, XUE Yan, ZENG Fanyi

图2 DUX诱导的2CLC的发育潜能的检测 Note： A. RT-qPCR validation of selected pluripotent marker genes in noDox (control) and plusDox (DUX overexpression) ES cells. B/C. Heatmap representation of expression levels of genes involved in 2CLCs (B) or endoderm (C) specification in noDox (control) and plusDox (DUX overexpression) cells. D. RT-qPCR was employed to assess the RNA expression levels of marker factors for endoderm, mesoderm and ectoderm on the fourth day of embryoid body differentiation in noDox (control) and plusDox (DUX overexpression) cells. ①P>0.05, ②P<0.001, ③P=0.001 3, compared with the control group.

Fig 2 Detection of developmental potential of DUX-induced 2CLCs