双同源盒诱导小鼠胚胎干细胞向胚外内胚层分化的机制研究

洪磊, 郭传亮, 蔡勤, 李婉睿, 曾溢滔, 薛燕, 曾凡一

Mechanism of DUX-induced differentiation of mESC into extraembryonic endoderm

HONG Lei, GUO Chuanliang, CAI Qin, LI Wanrui, ZENG Yitao, XUE Yan, ZENG Fanyi

表1 用于RT-qPCR的引物序列

Tab 1 Primer sequences used for RT-qPCR

Gene Forward primer (5′→3′) Reverse primer (5′→3′) Gapdh AGGTCGGTGTGAACGGATTTG TGTAGACCATGTAGTTGAGGTCA Dux ACTTCTAGCCCCAGCGACTC CCATGCTGCCAGGATTTCTA Zscan4c GTCCTGACAGAGGCCTGCC GAGATGTCTGAAGAGGCAAT Zfp352 AAGTCCCACATCTGAAGAAACAC GGGTATGAGGATTCACCCACA MERVL-pol ATCTCCTGGCACCTGGTATG AGAAGAAGGCATTTGCCAGA Nanog TCTTCCTGGTCCCCACAGTTT GCAAGAATAGTTCTCGGGATGAA Sox2 GCGGAGTGGAAACTTTTGTCC CGGGAAGCGTGTACTTATCCTT Klf4 GTGCCCCGACTAACCGTTG GTCGTTGAACTCCTCGGTCT Oct4 GGCTTCAGACTTCGCCTCC AACCTGAGGTCCACAGTATGC Gata4 CCCTACCCAGCCTACATGG ACATATCGAGATTGGGGTGTCT Gata6 GAGCTGGTGCTACCAAGAGG TGCAAAAGCCCATCTCTTCT Sox17 GAATTTCCATCTCCACCTCCG CGATTGGCACCTTTCACCC Nestin CTGCAGGCCACTGAAAAGTT GACCCTGCTTCTCCTGCTC Tubb3 CGGCAACTATGTAGGGGACT CCAGCACCACTCTGACCAA Hand1 AAGGATGCACAAGCAGGTGAC TTTAATCCTCTTCTCGCCGGG Myod1 CCACTCCGGGACATAGACTTG AAAAGCGCAGGTCTGGTGAG Flk1 CCTGGTCAAACAGCTCATCA AAGCGTCTGCCTCAATCACT