单细胞基因组学比较人诱导多能干细胞来源巨噬细胞与外周血来源巨噬细胞的异质性

张羽桐, 侯国俊, 沈南

Comparison of human-induced pluripotent stem cell-derived macrophages with peripheral blood-derived macrophages using single-cell genomics

ZHANG Yutong, HOU Guojun, SHEN Nan

图1 iPSC分化为IPSDM的过程及鉴定 Note： A. Flowchart of iPSCs differentiation into IPSDMs. B. Morphology of iPSCs used for differentiation (×100). C. Morphology of iPSCs on the 3rd day of differentiation into EBs (×40). D. Morphology of EBs on the 7th day of differentiation into monocytes (×100). E. Morphology of cells in the medium on the 21st day of EB differentiation into monocytes (×100). F. Flow cytometry analysis results of CD14 marker expression in IPSDMs. G. RT-qPCR results of HLA-DRA, CD68, CD86 and MRC1 gene expression levels in IPSDMs and U-937 cells.

Fig 1 Process and identification of iPSCs differentiation into IPSDMs