GPR87通过激活RHO/ROCK通路促进非小细胞肺癌的侵袭和迁移
GPR87 promotes invasion and migration through the RHO/ROCK pathway in non-small cell lung cancer
Note： A. The mRNA expression of MMP2, MMP7 and MMP9 in SK-MES-1 cells treated with siRNA-GPR87 was decreased, as detected by RT-qPCR. B. The protein expression of MMP7 in the supernatant of SK-MES-1 and A549 cells treated with siRNA-GPR87 was decreased, as detected by ELISA. The protein expression of active-MMP9 and GPR87 was decreased in SK-MES-1 (C) and A549 cells (D) treated with siRNA-GPR87, as detected by Western blotting. E. The mRNA expression of MMP2, MMP7 and MMP9 was increased in A549 cells treated with pCMV-GPR87-his, as detected by RT-qPCR. F. The protein expression of MMP7 in the supernatant of SK-MES-1 and A549 cells treated with pCMV-GPR87-his was increased, as detected by ELISA. The protein expression of active-MMP9 and GPR87 was increased in A549 (G) and SK-MES-1 cells (H) treated with pCMV-GPR87-his, as detected by Western blotting. Error bars represent the x±s. ①P<0.001, ②P=0.008, ③P=0.013,④P=0.041, ⑤P=0.009, ⑥P=0.043, compared with the NC group. ⑦P=0.007, ⑧P<0.001, ⑨P=0.003, ⑩P=0.002, 11P=0.005, 12P=0.008, 13P=0.001, 14P=0.021, compared with the Vector group.
