GPR87通过激活RHO/ROCK通路促进非小细胞肺癌的侵袭和迁移

刘晨茜, 韩林, 杨轶, 周韩, 刘亚云, 盛德乔

GPR87 promotes invasion and migration through the RHO/ROCK pathway in non-small cell lung cancer

LIU Chenxi, HAN Lin, YANG Yi, ZHOU Han, LIU Yayun, SHENG Deqiao

表2 RT-qPCR引物序列

Tab 2 Sequences of primers for RT-qPCR

Gene Forward (5'→3') Reverse (5'→3') GPR87 GGAGGCGACATCAATGCAG AAATGAAAGTAAAGAACGATTTTGTGT MMP2 GCTGGAGACAAATTCTGGAGATACA GTATCGAAGGCAGTGGAGAGGA MMP7 GAGTGAGCTACAGTGGGAACA CTATGACGCGGGAGTTTAACAT MMP9 GCCACTACTGTGCCTTTGAGTC CCCTCAGAGAATCGCCAGTACT E-cadherin GCCTCCTGAAAAGAGAGTGGAAG TGGCAGTGTCTCTCCAAATCCG N-cadherin CCTCCAGAGTTTACTGCCATGAC GTAGGATCTCCGCCACTGATTC vimentin AGGCAAAGCAGGAGTCCACTGA ATCTGGCGTTCCAGGGACTCAT snail TGCCCTCAAGATGCACATCCGA GGGACAGGAGAAGGGCTTCTC twist GCCAGGTACATCGACTTCCTCT TCCATCCTCCAGACCGAGAAGG RHOA GCAGGTAGAGTTGGCTTTATGG CTTGTGTGCTCATCATTCCGA RHOC AAGACGAGCACACCAGGAGAGA TTGGCTGAGCACTCAAGGTAGC ROCK1 GAAACAGTGTTCCATGCTAGACG GCCGCTTATTTGATTCCTGCTCC β-actin CTGGAACGGTGAAGGTGACA AAGGGACTTCCTGTAACAACGCA