荧光转基因斑马鱼品系Tg （amh：mCherry）的构建及其性腺发育的示踪分析

吴婧, 徐悦, 凌诗颖, 宋怀东, 乔洁, 董梅

Construction of fluorescent transgenic zebrafish Tg(amh:mCherry) and tracer analysis of its gonadal development

WU Jing, XU Yue, LING Shiying, SONG Huaidong, QIAO Jie, DONG Mei

图 2 F3代转基因斑马鱼的幼鱼、成鱼精巢中 mCherry 的表达位置和内源性 amh 表达位置的一致性分析 Note：A. In situ hybridization results of endogenous amh in 30dpf transgenic juvenile zebrafish. B/C. Bright-field observation (B) and mCherry fluorescence observation (C) of 30dpf transgenic juvenile zebrafish. D/E. In situ hybridization results of endogenous amh (D) and mCherry (E) in the spermatheca of adult transgenic zebrafish.

Fig 2 Consistency analysis of mCherry and endogenous amh expression sites in the F3 transgenic juvenile zebrafish and the spermatheca of adult transgenic zebrafish