基于单细胞测序技术解析冠状动脉粥样硬化患者平滑肌细胞的细胞间通信及关键基因

司春婴, 王建茹, 李晓辉, 王永霞, 关怀敏

Study on intercellular communication and key genes of smooth muscle cells in human coronary atherosclerosis based on single cell sequencing technology

SI Chunying, WANG Jianru, LI Xiaohui, WANG Yongxia, GUAN Huaimin

表1 RT-PCR引物序列

Tab 1 Primer sequence for RT-PCR

Primer Sequence (5'→3') Length/bp ITGB2-F ACCTCATGGATCTCTCCTACTC 274 ITGB2-R CCGACCTCTGTCTGAAACTG PTPRC-F AGTCTCTACGCAAAGCACGG 254 PTPRC-R AGCACTATTGGTAGGCTCCG CCL2-F GCTGACCCCAAGAAGGAATG 183 CCL2-R TGAGGTGGTTGTGGAAAAGG DCN-F AATCCCTTATGACCCTGACA 275 DCN-R TTTCCAACTTCACGAGAGGT IGF1-F GATGCTCTTCAGTTCGTGTG 259 IGF1-R GCTTCGTTTTCTTGTTTGTC GAPDH-F CCTTCCGTGTTCCTAC 152 GAPDH-R GACAACCTGGTCCTCA