KHSRP通过ANK3调节前列腺癌细胞对雄激素的反应性

蔡人杰, 徐明

KHSRP regulates the responsiveness of prostate cancer cells to androgens through ANK3

CAI Renjie, XU Ming

图4 LNCaP细胞中 KHSRP 所调控基因的检测
Note： A. Heatmap of differentially expressed genes between LNCaP^sh-^KHSRP^{and LNCaP^sh-^KHSRP^-^HA^-^KHSRP stable cell lines based on RNA-seq. B. Bubble plot of KEGG enrichment analysis of differential genes between LNCaP^sh-^KHSRP and LNCaP^sh-^KHSRP^-^HA^-^KHSRP stable cell lines from RNA-seq. C. Bar chart showing the expression level of ANK3 in LNCaP^sh-^KHSRP and LNCaP^sh-^KHSRP^-^HA^-^KHSRP stable cell lines based on RNA-seq. FPKM—fragments per kilobase of transcript per million mapped reads. D. Detection of ANK3 mRNA expression in stable LNCaP cell lines through RT-qPCR. E. Detection of ANK3 protein expression in stable LNCaP cell lines through Western blotting. F. Analysis of the correlation between ANK3 and KHSRP expression levels in prostate cancer using the TCGA database.^①P=0.010, ^②P=0.001.}

Fig 4 Detection of genes regulated by KHSRP in LNCaP cells