KHSRP通过ANK3调节前列腺癌细胞对雄激素的反应性
蔡人杰, 徐明
KHSRP regulates the responsiveness of prostate cancer cells to androgens through ANK3
CAI Renjie, XU Ming
表 1
慢病毒质粒构建所用引物序列
Tab 1
Primer sequences used for lentiviral vector plasmid construction
Primer
Sequence (5′→3′)
sh-
KHSRP
-UTR-F
GATCCGCTGAGGATAAAGCAATTCATTCTCGAGAATGAATTGCTTTATCCTCAGCTTTTTG
sh-
KHSRP
-UTR-R
AATTCAAAAAGCTGAGGATAAAGCAATTCATTCTCGAGAATGAATTGCTTTATCCTCAGCG
sh-
KHSRP
-CDS-F
GATCCGCCCGAGAAGATTGCTCATATACTCGAGTATATGAGCAATCTTCTCGGGCTTTTTG
sh-
KHSRP
-CDS-R
AATTCAAAAAGCCCGAGAAGATTGCTCATATACTCGAGTATATGAGCAATCTTCTCGGGCG
HA-KHSRP
-F
TTTTGACCTCCATAGAAGATTATGGGTACCTATCCTTACGA
HA-KHSRP
-R
GCGTCTAGGAAGAGCTCGATCAGTTAGAGGAGAACTTGTCT