3种灵长类动物SAGE1与INTS3相互作用保守性的验证

李晓畅, 李明月, 雷鸣, 武健

Conservation of protein interaction between SAGE1 and INTS3 identified in 3 different types of primates

LI Xiaochang, LI Mingyue, LEI Ming, WU Jian

图2 SAGE1与INTS3的免疫共沉淀实验、蛋白纯化及解离常数测定 Note： A. Immunoprecipitation of full-length INTS3 and full-length SAGE1 in Rhesus. B. Immunoprecipitation of full-length INTS3 and full-length SAGE1 in Marmoset. C. Immunoprecipitation of full-length INTS3 and full-length SAGE1 in Lemur. D. Purification of human INTS3CTD. E. Purification of Rhesus SAGE1CTD. Gel filtration chromatography results shown on the left; SDS-PAGE analysis of fractions across the major peak shown on the right. F. Purification of Marmoset SAGE1CTD. G. Purification of Lemur SAGE1CTD. H. Rhesus SAGE1CTD binding to INTS3CTD in a concentration-dependent manner with a slow kinetics. I. Marmoset SAGE1CTD binding to INTS3CTD in a concentration-dependent manner with a slow kinetics. J. Lemur SAGE1CTD binding to INTS3CTD in a concentration-dependent manner with a slow kinetics. Co-IP—Co-immunoprecipitation; SPR—surface plasmon resonance.

Fig 2 Co-immunoprecipitation experiment, protein purification and dissociation constant determination of SAGE1 and INTS3