阻塞性睡眠呼吸暂停的动物模型研究进展
沈煜斌, 欧茜文, 刘松

Progress in animal model research on obstructive sleep apnea
SHEN Yubin, OU Xiwen, LIU Song
表1 OSA模型优缺点对比
Tab 1 Comparison of advantages and disadvantages of each OSA model
ClassificationNameAdvantageLimitationApplicable scenario
Natural OSA animal modelObese pigs, English bulldogs, Obese Zucker rats, New Zealand Obese miceNo intervention required; closer to actual conditionsDifficult to fully control variables, adjust research conditions, and cover features closely related to unique human lifestyles and environmental factorsStudying the physiological, genetic, metabolic impacts and the long-term effects of chronic OSA
Direct OSA animal modelOSA animal model with tracheal intubationConsistently inducing the same degree of upper airway narrowing; easy to control the frequency and duration of apnea eventsTechnical difficulty and high cost; invasive models are prone to stress responses, and the airway obstruction plane differs from humansStudying the acute physiological effects of short-term OSA; not suitable for large-scale studies
OSA animal model of airway collapse induced by negative pressureSimple, stable, and repeatable operation; no need for complex surgeries or equipmentUnable to stably induce the same degree of upper airway collapseResearching the chronic impacts of long-term OSA, such as the development of cardiovascular diseases
OSA animal model with filling material injectionRelatively simple operation; spontaneous chronic IH pathophysiological changes occur; imaging can be used as supportAnimals show individual differences, poor repeatability; potential for injection site infectionsStudying the effects of anatomical and functional changes in the upper airway on OSA
Chemically induced OSA animal modelMinimally invasive, few surgery-related complications; high repeatabilityHigh technical requirements and complexity in operation; requiring precise and skilled experimental techniques and high-end experimental equipmentResearching the pathophysiological characteristics of OSA during specific sleep stages
Special mask ventilation blockage method, Head-enclosed ventilation blockage method, oral-nasal airbag ventilation blockage methodControllable ventilation and occlusion timesHigh equipment requirements; difficult operation; insufficient precision in control; restricting animal free movementNot suitable for more complex or larger-scale studies
Indirect OSA animal modelChronic IH modelNo anesthesia required; non-invasive; precise control of gas concentrations within the chamber; safe and repeatable modeling processUnable to simulate upper airway narrowing or collapse; CO2 partial pressure changes do not match actual conditionsSuitable for mechanistic research, long-term studies, and large-scale physiological and pharmacological research
Sleep deprivation modelAbility to adjust the intensity and duration of sleep deprivation as needed; facilitating a repeatable process

Introduction of additional stress factors; possibility of leading to

a certain degree of exercise deprivation effect, confinement, and fixation stress

Researching the impact of decreased sleep quality and disordered sleep structure on cognitive function, learning and memory, emotional regulation, and metabolic diseases in OSA, and the treatment effects of improving sleep quality on these diseases