SAE1与SAE2蛋白相互作用肽抑制剂的多种体外筛选体系的构建与评价

胡晨阳, 陆绍永, 杨秀岩

Establishment and evaluation of various in vitro screening systems for peptide inhibitors targeting SAE1 and SAE2 interaction

HU Chenyang, LU Shaoyong, YANG Xiuyan

图4 基于SAE酶活的筛选体系的建立 Note： A. Effect of the absence of SAE1 or SAE2 on the adenylation efficiency of SAE. B. The adenylation efficiencies of SAE under different concentrations of SUMO1. C. Optimal concentration of SAE; ΔRLU=RLUcontrol－RLUsample. D. Optimal concentration of SUMO1. E. Inhibition curve of COH000. F. Inhibition rates of peptides at 125 μmol·L-1. G. Inhibition curve of HP1B. H. Binding interaction between HP1B (blue) and SAE1 (gray). Key interacting residues of interaction are shown as sticks and labeled. Yellow dashed lines represent hydrogen bonds.

Fig 4 Establishment of a SAE enzyme activity-based screening system