SAE1与SAE2蛋白相互作用肽抑制剂的多种体外筛选体系的构建与评价
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胡晨阳, 陆绍永, 杨秀岩
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Establishment and evaluation of various in vitro screening systems for peptide inhibitors targeting SAE1 and SAE2 interaction
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HU Chenyang, LU Shaoyong, YANG Xiuyan
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表1 PCR引物序列
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Tab 1 Primer sequences for PCR
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| Primer | Forward sequence (5′→3′) | Reverse sequence (5′→3′) |
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| SAE1 | GCAAATGGGTCGCGGATCCGAATTCATGGTGGAGAAGGAGGAG | GTGGTGCTCGAGTGCGGCCGCATCACTTGGGGCCAAGG | | SAE2 | GCAAATGGGTCGCGGATCCGAATTCATGGCACTGTCGCG | GTGGTGCTCGAGTGCGGCCGCATCAATCTAATGCTATGAC | | SUMO1 | GCGCGGCAGCCATATGGCTAGCATGTCTGACCAGGAGGC | GGTGGTGGTGGTGGTGCTCGAGTTAACCCCCCGTTTGTTCC |
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