SAE1与SAE2蛋白相互作用肽抑制剂的多种体外筛选体系的构建与评价

胡晨阳, 陆绍永, 杨秀岩

Establishment and evaluation of various in vitro screening systems for peptide inhibitors targeting SAE1 and SAE2 interaction

HU Chenyang, LU Shaoyong, YANG Xiuyan

表3 不同蛋白使用的缓冲液

Tab 3 Buffers for different proteins

Buffer Component Tris-HCl (pH 8.0)/(mmol·L-1) NaCl/(mmol·L-1) Imidazole/(mmol·L-1) DTT/(mmol·L-1) SAE1 lysis buffer 50 300 10 1 SAE1 Ni-NTA equilibration/wash buffer 50 50 20 1 SAE1 Ni-NTA elution buffer 50 50 40 1 SAE2 lysis buffer 50 300 / 1 SAE2 Ni-NTA equilibration/wash buffer 50 50 / 1 SAE2 Ni-NTA elution buffer 50 50 25 1 SAE2 anion exchange buffer A 50 50 / 1 SAE2 anion exchange buffer B 50 1 / 1 SUMO1 lysis buffer 50 500 20 / SUMO1 Ni-NTA equilibration/wash buffer 50 500 20 / SUMO1 Ni-NTA elution buffer 50 50 400 /