成骨细胞条件性视黄酸信号失活小鼠模型的构建与验证

孙思远, 刘媛琪, 崔怡雯, 黄紫晗, 梅李, 代庆刚, 江凌勇

Generation and validation of the conditional osteoblast-specific retinoic acid signaling inhibition mouse model

SUN Siyuan, LIU Yuanqi, CUI Yiwen, HUANG Zihan, MEI Li, DAI Qinggang, JIANG Lingyong

图1 Cre-LoxP系统实现 dnRARα 基因条件性表达的原理示意图 Note： A. Schematic diagram of the structure of the dominant negative protein dnRARα. B. Schematic diagram of the Cre-LoxP system in Osx+ cell lineage of OsxCre;Rosa26dn/dn mice. C. Schematic diagram of the activity of retinoic acid signaling in Osx+ cell lineage of OsxCre mice and OsxCre;Rosa26dn/dn mice. AF1—activation function 1; AF2—activation function 2; DBD—DNA binding domain; LBD—ligand binding domain; prom.—promoter.

Fig 1 Schematic diagram of conditional dnRARα gene expressing by Cre-LoxP system