成骨细胞条件性视黄酸信号失活小鼠模型的构建与验证

孙思远, 刘媛琪, 崔怡雯, 黄紫晗, 梅李, 代庆刚, 江凌勇

Generation and validation of the conditional osteoblast-specific retinoic acid signaling inhibition mouse model

SUN Siyuan, LIU Yuanqi, CUI Yiwen, HUANG Zihan, MEI Li, DAI Qinggang, JIANG Lingyong

图3 OsxCre;Rosa26dn/dn 小鼠成骨细胞视黄酸信号失活效率验证 Note： A. The RARα protein level of femur bone BMSCs after osteogenic induction from OsxCre mice and OsxCre;Rosa26dn/dn mice by Western blotting. B. The RARα protein level of parietal bone cells after osteogenic induction from OsxCre mice and OsxCre;Rosa26dn/dn mice by Western blotting. C. The RARE activity of parietal bone cells from OsxCre mice and OsxCre;Rosa26dn/dn mice by dual luciferase gene reporter assay. PGL3-basic is blank plasmid group. OBS—osteoblast; ns—no significant difference.

Fig 3 Verification of the inhibition of osteoblastic retinoic acid signaling in OsxCre;Rosa26dn/dn mice