低氧诱导的长链非编码RNA 68在肝癌中的功能及其作用机制
Function and mechanism study of hypoxia-induced long non-coding RNA 68 in hepatocellular carcinoma
Note： A. Western blotting analysis of SMMC-7721 cells indicated the knockdown efficiency of HIF1α/HIF2α/HIF1β. B. qRT-PCR analysis of indicated RNAs of SMMC-7721 cells with or without knockdown HIF1α/HIF2α/HIF1β under hypoxia. The P values indicate the differences between each group and the control group (shNC). C. Western blotting analysis of PLC/PRF/5 cells indicated the knockdown efficiency of HIF1α/HIF2α/HIF1β. D. qRT-PCR analysis of indicated RNA of PLC/PRF/5 cells with or without knockdown HIF1α/HIF2α/HIF1β under hypoxia. The P values indicate the differences between each group and the control group (shNC). E. Reference sequence diagram of HILRNA68. TSS—transcription start site. F. Western blotting analysis of HEK-293T cells transfected with HIF1α over-expression plasmid. G. Dual-luciferase assay results of HEK-293T transfected with HIF1α over-expression plasmid. H/N—hypoxia/normoxia.
