| Tumor wash buffer | |
| PBS (1×) | Up to 20 mL (for 1 g biopsies) |
| DTT (2 mol·L-1) | 65 μL (final 6.5 mmol·mL-1) |
| FBS | 2 mL (10%) |
| Normal wash buffer | |
| PBS (1×) | Up to 20 mL (for 1 g biopsies) |
| HEPES (1 mol·L-1) | 300 μL (final 15 mmol·mL-1) |
| EDTA (0.5 mol·L-1) | 200 μL (final 5 mmol·mL-1) |
| DTT (2 mol·L-1) | 10 μL (final 1 mmol·mL-1) |
| FBS | 2 mL (10%) |
| Tissue digestion buffer | |
| RPMI1640 (+10%FBS, +1%P/S) | Up to 40 mL (for 2 g biopsies) |
| Col Ⅷ | 45.7 μL (final 0.38 mg·mL-1) |
| DNase Ⅰ | 26.8 μL (final 0.1 mg·mL-1) |