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Analysis of hair follicle microbiota in non-lesional areas of patients with moderate-to-severe acne vulgaris: a single-center cross-sectional study

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表2 qPCR绝对定量程序

Tab 2 qPCR absolute quantification program

Step Program Step 1 95 ℃ 30 s Step 2 (40 cycles) 95 ℃ 5 s; 55 ℃ 30 s; 72 ℃ 30 s Step 3 (dissociation stage) 94 ℃ 30 s; 70 ℃ 1.5 min; 94 ℃ 10 s