预免疫策略结合mVenus-p27K-系统构建休眠肿瘤小鼠模型
木司塔巴·木台力甫, 王俊杰, 钱云臻, 陈溯源, 邵达, 张志刚, 李冬雪

A dormant cancer mouse model established by combining preimmune strategy with mVenus-p27K - system
MUTAILIFU Musitaba, WANG Junjie, QIAN Yunzhen, CHEN Suyuan, SHAO Da, ZHANG Zhigang, LI Dongxue
表1 qPCR引物序列
Tab 1 Primer sequences for qPCR
GeneForward (5′→3′)Reverse (5′→3′)
Kdm7aGATAAGTAATGGCAGCCTGAGCAGCTCTGTCACACGAAGAAG
Kdm5bAATACCTGGATAGTGGCTGGTCCAGTGGTCTTCAATGTGC
Kdm6bGTCCATTGTGCCCATGATTCGTCGCATTCGTTGCAGTAGTAG
Pdcd4AAGCGGAAAGACAGTGTGTGGGCTTCATATACAAGCTCGTGG
Tob1TCCTGAAGCAGAAAGCCATCTTGGCATTGGGAGAAAGAGC
Idh1CATGTACCAGAAAGGGCAAGAGTAATGCAAGCAGCCAAGTCC
Ddit4ACCTTTCAGTTGACCCTGGTGTGTAACCAGGGACCAAGGAAG
Nrf2CGAGATATACGCAGGAGAGGTAAGCAGTAGATGGAGGTTTCTGTCG
Ypel2CAGTAGTTAATGTGGGCTGTGGTGGATGCTATCAGGTCAGTC
Mki67CCTTGGCTTAGGTTCACTGTCCTGCAGAATCCAGATGATGGAGC
β-actinGGCTGTATTCCCCTCCATCGCCAGTTGGTAACAATGCCATGT