七氟烷对原代少突胶质细胞增殖和分化的影响
Effects of sevoflurane exposure on proliferation and differentiation of primary oligodendrocytes
Note： A. Immunofluorescence staining showed the expression of MBP in oligodendrocytes. The scale bar is 100 μm. B. The number of Hoechest-positive cells normalized the number of MBP-positive cells. Multiple sevoflurane exposures significantly reduced the number of MBP-expressing cells. C. Immunofluorescence staining showed the expression of MAG in oligodendrocytes. The scale bar is 100 μm. D. The number of MAG-positive cells was normalized by the number of Hoechest-positive cells. Multiple sevoflurane exposures significantly reduced the number of MAG-expressing cells. E. Detection of MBP expression levels in oligodendrocytes by Western blotting. F. Western blotting results showed that multiple sevoflurane exposures significantly reduced the expression of MBP protein levels. ①P=0.030, ②P=0.026, ③P=0.001, compared with the control group.
