七氟烷对原代少突胶质细胞增殖和分化的影响
Effects of sevoflurane exposure on proliferation and differentiation of primary oligodendrocytes
Note： A/B. CCK8 results suggested that sevoflurane had a killing effect on OPCs. A. 1, 2, and 3 treatments with sevoflurane all had a killing effect on OPCs. As the number of sevoflurane exposures increased, the survival rate of OPCs decreased. B. The survival rate of OPCs decreased significantly on day 1, 2, and 3 after sevoflurane exposure, but there was no significant difference between each detection day. C. Detection of caspase-3 expression levels in OPCs by Western blotting. D. Western blotting showed that multiple sevoflurane exposures increased the expression of caspase-3 protein levels. ①P=0.000, compared with the group with 0 sevoflurane exposure; ②P=0.020, compared with the group with 1 sevoflurane exposure; ③P=0.011, ④P=0.030, ⑤P=0.046, compared with the group without sevoflurane exposure; ⑥P=0.012, compared with the control group.
