七氟烷对原代少突胶质细胞增殖和分化的影响

施灵玲, 程燕咏, 张磊

Effects of sevoflurane exposure on proliferation and differentiation of primary oligodendrocytes

SHI Lingling, CHENG Yanyong, ZHANG Lei

图4 过表达YTHDF1对多次七氟烷处理引起OPC损伤的修复作用 Note： A. Detection of YTHDF1 expression levels in OPCs by Western blotting. B. Western blotting results showed that multiple sevoflurane exposures reduced the expression level of YTHDF1 protein. C. CCK8 experiments indicated that sevoflurane exposures killed OPCs and significantly reduced cell survival rate. However, cells overexpressing YTHDF1 had a partial protective effect on the killing effect of sevoflurane. ①P=0.011, compared with the control group; ②P=0.000, compared with the group without sevoflurane exposure and with control virus infection; ③P=0.007, compared with the group with 3 sevoflurane exposures and control virus infection.

Fig 4 Overexpression of YTHDF1 rescued the damage of OPCs caused by multiple sevoflurane exposures