非经典型多梳抑制复合物1.6的电镜结构分析

蔡单, 黄晶

Electron microscopic study of the non-canonical polycomb repressive complex 1.6

CAI Dan, HUANG Jing

图3 PRC1.6七元复合物的泛素化活性实验和EMSA Note： A. Ubiquitination assay of the PRC1.6 complex. The upper two figures are Western blotting analysis of ubiquitination assay, and the lower figure is SDS-PAGE of ubiquitination assay. B. EMSA of the PRC1.6 complex. The upper figure on the left is Native-PAGE analysis of EMSA, which was stained by Coomassie brilliant blue in the lower figure. The right figure is SDS-PAGE analysis of EMSA input. * denotes TRIM28 and ** denotes HSP70. Ub—ubiquitin; H2Aub—H2A with one ubiquitin; H2Aub2—H2A with two ubiquitins.

Fig 3 Ubiquitination assay and EMSA of the PRC1.6 heptameric complex