肿瘤坏死因子-α在新型冠状病毒感染合并肾损伤中的作用

PANDIT Roshan, 卢君瑶, 何立珩, 包玉洁, 季萍, 陈颖盈, 许洁, 王颖

Role of tumor necrosis factor-α in coronavirus disease 2019-associated kidney injury

PANDIT Roshan, LU Junyao, HE Liheng, BAO Yujie, JI Ping, CHEN Yingying, XU Jie, WANG Ying

图4 经S1蛋白处理的THP-1细胞培养上清可诱导HK-2细胞的炎性特征 Note： A. Diagram of the culture of HK-2 cells in conditioned medium for 24 h. Conditioned medium was obtained by stimulating THP-1 cells with S1 spike protein (500 ng/mL) for 4 h and 8 h. B. Fold changes in TNF-α mRNA levels in HK-2 cells cultured in conditioned medium compared to control HK-2 cells. C. Fold changes in CXCL10 mRNA levels in HK-2 cells cultured in conditioned medium compared to control HK-2 cells. ①P=0.005, compared with the control; ②P=0.225, compared with the HK-2+4 h sup; ③P=0.324, compared with the control; ④P=0.001, compared with the HK-2+4 h sup.

Fig 4 Inflammatory properties of HK-2 cells induced by the supernatants from S1 protein-treated THP-1 cells