基于多尺度分辨率的耳蜗神经纤维三维成像

成亚琼, 杜一唯, 刘思迪, 经典, 吴皓

Three-dimensional imaging of cochlear nerve fibers based on multi-scale resolution

CHENG Yaqiong, DU Yiwei, LIU Sidi, JING Dian, WU Hao

图4 耳蜗内单根神经纤维追踪结果及2种神经纤维的路径差异 Note： A. 3D images of local spiral ganglia and fibers at a resolution of 0.24 μm×0.24 μm×1.50 μm. B/C. Two types of fiber pathways obtained by Imaris software tracing, an auditory nerve fiber (B) and MOC/LOC fibers (C). D. Local magnification of the 3D imaging of cochlear and labelling of fiber tracing at high resolution. E/F. Comparison of fiber length (E) and path curvature (F) between localized auditory nerve fibers and the MOC/LOC fibers within the cochlea. Neuronal designations were used to represent their corresponding fibers, with SGNs referring to auditory nerve fibers and OGNs (olivocochlear neurons) referring to MOC/LOC fibers. The orange/yellow dots represent the cell bodies of the tracked auditory nerves in B and D. ①P=0.002, ②P=0.113.

Fig 4 Single nerve fiber tracing in the cochlea and differences in pathways characteristics between two types of neural fibers