血红蛋白诱导心肌细胞铁死亡及Basigin的调控机制研究

李文丽, 钟方元, 赵怡超, 金力行, 雷杰, 石瑶, 卜军, 葛恒

Mechanisms of Basigin regulation in hemoglobin-induced cardiomyocyte ferroptosis

LI Wenli, ZHONG Fangyuan, ZHAO Yichao, JIN Lixing, LEI Jie, SHI Yao, PU Jun, GE Heng

图5 敲低 Bsg 对Hb诱导的H9c2心肌细胞损伤的影响 Note： A. Western blotting analysis of BSG protein expression levels after si-NC or si-Bsg transfection. B. Real-time quantitative PCR analysis of Bsg mRNA expression levels after si-NC or si-Bsg transfection. C. Flow cytometry analysis of the effect of Bsg silencing on the PI-positive rate in Hb-treated H9c2 cardiomyocytes. D. Quantitative analysis of the flow cytometry results. E. WST-1 assay to detect the viability of H9c2 cardiomyocytes in the indicated groups. F. Real-time quantitative PCR analysis of Ptgs2 mRNA expression in Hb-treated H9c2 cardiomyocytes after Bsg silencing. ①P<0.001, ②P=0.011.

Fig 5 Effects of knocking down Bsg on Hb-induced H9c2 cardiomyocyte injury