人胚胎干细胞向神经前体细胞分化的转录组与染色质可及性变化分析研究

李林颖, 蔡晓东, 童冉, 杨晨, 王志明, 贺潇宇, 马子越, 张丰, 李令杰, 周君梅

Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells

LI Linying, CAI Xiaodong, TONG Ran, YANG Chen, WANG Zhiming, HE Xiaoyu, MA Ziyue, ZHANG Feng, LI Lingjie, ZHOU Junmei

图3 NPC分化过程中转录组的分析 Note： A. Volcano plot shows the distribution of DEGs between the two distinct developmental stages (ESC and NPC). DEGs were defined as |log2FC|>1 and P<0.05. B. Gene expression levels were clustered across all samples. Different colors indicate the value of each gene expression level normalized with Z-scores. C. GO analysis was performed on the DEGs upregulated at the NPC stage. D. KEGG analysis of the DEGs upregulated at NPC stage. E. GSEA analysis identifies pathways upregulated during NPC differentiation.

Fig 3 Transcriptome analysis of NPC differentiation