人胚胎干细胞向神经前体细胞分化的转录组与染色质可及性变化分析研究

李林颖, 蔡晓东, 童冉, 杨晨, 王志明, 贺潇宇, 马子越, 张丰, 李令杰, 周君梅

Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells

LI Linying, CAI Xiaodong, TONG Ran, YANG Chen, WANG Zhiming, HE Xiaoyu, MA Ziyue, ZHANG Feng, LI Lingjie, ZHOU Junmei

图5 整合RNA-seq和ATAC-seq数据分析参与NPC分化的重要基因 Note： A. Venn diagram showing the overlap between DEGs in the NPC cell population and related genes predicted from differentially open chromatin regions. In the ATAC-seq dataset, genes were annotated to regions with ±1 kb of the promoter. B. Scatter plot showing the correlation between the expression levels of the overlapping genes (A) obtained in RNA-seq and the associated peaks obtained from ATAC-seq. C. GO analysis of 1 344 upregulated genes. D. Expression levels of CTNND2 and LHX2 in RNA-seq during NPC differentiation. E. The Integrative Genomics Viewer showing ATAC-seq signals at the transcription start sites of CTNND2 and LHX2 loci during NPC differentiation.

Fig 5 Integration of RNA-seq and ATAC-seq data to analyze important genes involved in NPC differentiation