人胚胎干细胞向神经前体细胞分化的转录组与染色质可及性变化分析研究

李林颖, 蔡晓东, 童冉, 杨晨, 王志明, 贺潇宇, 马子越, 张丰, 李令杰, 周君梅

Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells

LI Linying, CAI Xiaodong, TONG Ran, YANG Chen, WANG Zhiming, HE Xiaoyu, MA Ziyue, ZHANG Feng, LI Lingjie, ZHOU Junmei

表2 在神经诱导过程中RNA-seq文库所获得的原始读段数和比对读段数

Tab 2 Number of raw reads and mapped reads obtained from RNA-seq libraries during neural induction

Index Sample ESC_S1 ESC_S2 NPC_S1 NPC_S2 Total raw read pairs/n 45 792 974 53 251 136 50 419 620 52 973 024 Total raw bases/n 6 914 739 074 8 040 921 536 7 613 362 620 7 998 926 624 Mapped reads/n 44 506 927 51 742 966 48 948 795 51 574 332 Mapped ratio/% 97.95 97.92 97.87 98.13 High-quality mapped reads/n 43 139 843 50 148 322 47 576 740 50 093 517 High-quality mapped ratio/% 94.94 94.90 95.13 95.31