人胚胎干细胞向神经前体细胞分化的转录组与染色质可及性变化分析研究

李林颖, 蔡晓东, 童冉, 杨晨, 王志明, 贺潇宇, 马子越, 张丰, 李令杰, 周君梅

Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells

LI Linying, CAI Xiaodong, TONG Ran, YANG Chen, WANG Zhiming, HE Xiaoyu, MA Ziyue, ZHANG Feng, LI Lingjie, ZHOU Junmei

表3 在神经诱导过程中ATAC-seq文库所获得的原始读段数和比对读段数

Tab 3 Number of raw reads and mapped reads obtained by ATAC-seq libraries during neural induction

Index Sample ESC_S1 ESC_S2 NPC_S1 NPC_S2 Total raw read pairs/n 63 713 180 32 001 236 88 523 604 61 924 682 Total raw bases/n 9 620 690 180 4 832 186 636 13 367 000 000 9 350 626 982 Mapped read pairs/n 61 240 240 30 684 142 84 944 932 59 439 388 Mapped ratio/% 98.36 98.52 98.24 97.98 High-quality mapped read pairs/n 54 624 252 27 152 448 75 936 202 53 201 562 High-quality mapped ratio/% 87.73 87.18 87.82 87.70 Percent duplication/% 23.70 20.59 22.18 29.05 Non-duplicate read pairs/n 41 677 046 21 562 952 59 092 184 37 745 230