Fas相关死亡结构域蛋白促进头颈部鳞状细胞癌细胞增殖能力的机制研究

陈怡楠, 郑旸, 曾汉林, 雷鸣

Mechanism of Fas-associated protein with death domain in promoting proliferation of head and neck squamous cell carcinoma cells

CHEN Yinan, ZHENG Yang, ZENG Hanlin, LEI Ming

表1 shRNA及gRNA寡核苷酸序列

Tab1 Oligonucleotide sequences of shRNA and sgRNA

Name Sequence (5′→3′) shCON-F CCGGCCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGGTTTTTTG shCON-R AATTCAAAAAACCTAAGGTTAAGTCGCCCTCG CTCGAGCGAGGGCGACTTAACCTTAGG shFADD-1-F CCGGGTGCAGCATTTAACGTCATATCTCGAG ATATGACGTTAAATGCTGCACTTTTTTG shFADD-1-R AATTCAAAAAAGTGCAGCATTTAACGTCATATCTCGAGATATGACGTTAAATGCTGCAC shFADD-2-F CCGGGCTGACCTGGTACAAGAGGTTCTCGAGAACCTCTTGTACCAGGTCAGCTTTTTTG shFADD-2-R AATTCAAAAAAGCTGACCTGGTACAAGAGGTT CTCGAGAACCTCTTGTACCAGGTCAGC shFADD-3-F CCGGCATGGAACTCAGACGCATCTACTCGAGTAGATGCGTCTGAGTTCCATGTTTTTTG shFADD-3-R AATTCAAAAAACATGGAACTCAGACGCATCTACTCGAGTAGATGCGTCTGAGTTCCATG sgCUX1-F CACCGCCGGTTCGCCAATACCGTTG sgCUX1-R AAACCAACGGTATTGGCGAACCGGC