血液样本中DNA与RNA提取效率的研究

宿星蕾, 路平, 彭俊杰, 汪滋民, 宋萍, 韩达

Comparison of DNA and RNA extraction efficiency from blood

SU Xinglei, LU Ping, PENG Junjie, WANG Zimin, SONG Ping, HAN Da

图1 基于qPCR技术制作的2组标准曲线 Note： A. Standard curve plotted based on DNA concentration and CT value after qPCR reaction using amplicon 1 with an amplified DNA fragment length of 103 bp. B. Standard curve plotted based on DNA concentration and CT value after qPCR reaction using amplicon 2 with an amplified DNA fragment length of 71 bp. The correlation coefficients (R²) for the standard curves are close to 1 (R²≥0.999), indicating a strong linear relationship between logarithmic value of sample concentration (ng/μL) and CT signal intensity.

Fig 1 Two sets of standard curves based on qPCR technology