血液样本中DNA与RNA提取效率的研究

宿星蕾, 路平, 彭俊杰, 汪滋民, 宋萍, 韩达

Comparison of DNA and RNA extraction efficiency from blood

SU Xinglei, LU Ping, PENG Junjie, WANG Zimin, SONG Ping, HAN Da

图4 血浆cfDNA提取流程优化及100例结直肠癌样本血浆cfDNA提取 Note： A. Schematic of the optimized cfDNA extraction workflow from plasma by increasing the sample loading volume and extending the elution incubation time. B. Analysis of the results of cfDNA extraction using Kit B and optimized extraction process for sample 31 (initial input volumes: 0.3, 0.9, and 1.8 mL) from fibrosis patient and samples 32 and 33 (initial input volumes: 1.5 and 2.1 mL) from colorectal cancer patients. C. Plasma cfDNA content extracted from 100 colorectal cancer patient samples using Kit B.

Fig 4 Optimization of plasma cfDNA extraction process and plasma cfDNA extraction from 100 colorectal cancer samples