血液样本中DNA与RNA提取效率的研究

宿星蕾, 路平, 彭俊杰, 汪滋民, 宋萍, 韩达

Comparison of DNA and RNA extraction efficiency from blood

SU Xinglei, LU Ping, PENG Junjie, WANG Zimin, SONG Ping, HAN Da

图5 血浆中cfDNA受存放时间影响及扩增子长度对DNA分子捕获差异的比较 Note：A. cfDNA content in the first elution of colorectal cancer patient samples 32 and 33 at three time points (30 μL elution). B. cfDNA content in both the first and second elutions of colorectal cancer patient samples 32 and 33 at three time points (20 μL elution for the second). C. Schematic of amplicon design: amplicon 1 (103 bp) and amplicon 2 (71 bp). D. qPCR quantification of cfDNA from fibrosis patient sample 31 using the two amplicons (30 μL for the first elution, 20 μL for the second and third).

Fig 5 Comparison of the impact of storage time on plasma cfDNA and the difference in DNA molecule capture based on primer length