磷脂酰乙醇胺引起内质网应激促进巨噬细胞衰老及肝损伤

韩龙传, 李悦, 邹智慧, 罗静, 李若伊, 张颖婷, 唐欣欣, 田丽红, 陆宇恒, 黄莺, 贺明, 付寅坤

Phosphatidylethanolamine promotes macrophage senescence and liver injury by activating endoplasmic reticulum stress

HAN Longchuan, LI Yue, ZOU Zhihui, LUO Jing, LI Ruoyi, ZHANG Yingting, TANG Xinxin, TIAN Lihong, LU Yuheng, HUANG Ying, HE Ming, FU Yinkun

图1 PE促进巨噬细胞衰老及衰老相关分泌表型表达 Note： A. Representative images of SA-β-gal staining in RAW264.7 cells treated with different concentrations of PE (0, 10, 50 and 125 μmol·L-1) in the presence or absence of DOX (0.5 μmol·L-1), bar=50 μm. B/C. Quantitative real-time PCR analysis of expression of senescence markers p21 (B) and p16 (C) in RAW264.7 cells treated with different concentrations of PE (0, 10, 50 and 125 μmol·L-1) in the presence of DOX. D. Western blotting analysis of senescence markers. E‒I. Quantitative real-time PCR analysis of expression of senescence-associated secretory phenotype (SASP) factors Tnf-α (E), Il-6 (F), Il-1β (G), Cxcl1 (H) and Mmp13 (I). ①P<0.001, ②P=0.001, ③P=0.021, ④P=0.005, ⑤P=0.027, ⑥P=0.023, ⑦P=0.031, ⑧P=0.019, ⑨P=0.009, ⑩P=0.045, ⑪P=0.008, ⑫P=0.002. DOX—doxorubicin.

Fig 1 PE promotes cellular senescence and the expression of SASP in macrophages