磷脂酰乙醇胺引起内质网应激促进巨噬细胞衰老及肝损伤

韩龙传, 李悦, 邹智慧, 罗静, 李若伊, 张颖婷, 唐欣欣, 田丽红, 陆宇恒, 黄莺, 贺明, 付寅坤

Phosphatidylethanolamine promotes macrophage senescence and liver injury by activating endoplasmic reticulum stress

HAN Longchuan, LI Yue, ZOU Zhihui, LUO Jing, LI Ruoyi, ZHANG Yingting, TANG Xinxin, TIAN Lihong, LU Yuheng, HUANG Ying, HE Ming, FU Yinkun

图3 PE通过引起内质网应激信号通路促进巨噬细胞衰老和衰老相关分泌表型表达 Note：A‒C. Quantitative real-time PCR analysis of Ire1α (A), Atf6 (B) and Chop (C) mRNA expression in RAW264.7 cells treated with DOX and PE (50 μmol·L-1). D. Western blotting analysis of endoplasmic reticulum stress-related proteins, including p-IRE1α, IRE1α, ATF6-P, ATF6-N, XBP1s and ATF4. E. SA-β-gal staining of RAW264.7 cells treated with Toyocamycin, DOX and PE. F/G. Quantitative real-time PCR analysis of senescence marker mRNA expression, including p21 (F) and p16 (G). H. Western blotting analysis of proteins including p21 and p16. I‒M. Quantitative real-time PCR analysis of SASP mRNA expression, including Tnf-α (I), Il-6 (J), Il-1β (K), Cxcl1 (L) and Mmp13 (M). ①P=0.033, ②P=0.009, ③P<0.001, ④P=0.014, ⑤P=0.012, ⑥P=0.005, ⑦P=0.003, ⑧P=0.008, ⑨P=0.010.

Fig 3 PE promotes macrophage senescence and SASP through activating endoplasmic reticulum stress signaling pathway