基于纳米孔Cas9靶向测序的金黄色葡萄球菌快速检测与分型

潘抒凡, 杨栋磊, 王鹏飞

Rapid detection and typing of Staphylococcus aureus based on nanopore Cas9-targeted sequencing

PAN Shufan, YANG Donglei, WANG Pengfei

图3 Cas9 RNP切割体系优化 Note： A. Verification of cleavage products using the Cas9 RNP cleavage system (Cas9 RNP:dsDNA=1:2) at 20 ℃ for 60 min. B. Cleavage products verification of Cas9 RNP cleavage system (Cas9 RNP:dsDNA=1:1) with different cleavage time at 20 ℃. C. Cleavage efficiencies of Cas9 RNP under different ratios and different cleavage time.

Fig 3 Optimization of Cas9 RNP cleavage system