基于纳米孔Cas9靶向测序的金黄色葡萄球菌快速检测与分型

潘抒凡, 杨栋磊, 王鹏飞

Rapid detection and typing of Staphylococcus aureus based on nanopore Cas9-targeted sequencing

PAN Shufan, YANG Donglei, WANG Pengfei

图4 Cas9 RNP合成温度筛选 Note： A. Cleavage products of Cas9 RNP synthesized at 25 ℃, with different cleavage incubation times. B. Cleavage efficiencies of Cas9 RNP synthesized at 20 ℃ or 25 ℃, under different cleavage incubation times.

Fig 4 Screening of Cas9 RNP synthesis temperatures