基于纳米孔Cas9靶向测序的金黄色葡萄球菌快速检测与分型
潘抒凡, 杨栋磊, 王鹏飞

Rapid detection and typing of Staphylococcus aureus based on nanopore Cas9-targeted sequencing
PAN Shufan, YANG Donglei, WANG Pengfei
表1 模拟靶标双链DNA序列
Tab 1 Simulated double-stranded target DNA sequences
crRNAStrandCorresponding simulated target DNA (5'→3')
spa-fw1-crRNA+ACCAAATACAGTTGTACCGATGAATGGATTTTCTTCACCAGT
-ACTGGTGAAGAAAATCCATTCATCGGTACAACTGTATTTGGT
spa-fw2-crRNA+TGCAATGTCATTTACTGTATCACCAGGTTTAACGACATGTAC
-GTACATGTCGTTAAACCTGGTGATACAGTAAATGACATTGCA
spa-rev1-crRNA+GTTACAGATGCAATACCTACACCTAGTTTACGAATTGAATAA
-TTATTCAATTCGTAAACTAGGTGTAGGTATTGCATCTGTAAC
spa-rev2-crRNA+GCAGCAGGTGTTACGCCACCAGATATAAGTAATGTACCTAAA
-TTTAGGTACATTACTTATATCTGGTGGCGTAACACCTGCTGC
SCC2-fw1-crRNA+ATTAATCGTATTAATAGAATACAAGGGCAACTAAATGGAATT
-AATTCCATTTAGTTGCCCTTGTATTCTATTAATACGATTAAT
SCC2-fw2-crRNA+TAATAGAATGTGTAAAAGTAGCTGCGGATGATGAAGAAAGCT
-AGCTTTCTTCATCATCCGCAGCTACTTTTACACATTCTATTA
SCC2-rev1-crRNA+AATTCTTGGGCCAATCCTTCGGAAGATAGCATCTTTCCTTGT
-ACAAGGAAAGATGCTATCTTCCGAAGGATTGGCCCAAGAATT
SCC2-rev2-crRNA+TACAAAGTCGCTTTGCCCTTGGGTCATGCGTTGGTTCAATTC
-GAATTGAACCAACGCATGACCCAAGGGCAAAGCGACTTTGTA