去泛素化酶USP38通过稳定RBM14促进胃癌进展的机制研究

陈赟琪, 那迪娜·帕尔哈提, 张鹏善, 黄陈

Deubiquitinase USP38 promotes gastric cancer progression by stabilizing RBM14

Chen Yunqi, Paerhati Nadina, Zhang Pengshan, Huang Chen

图7 USP38通过稳定RBM14蛋白对胃癌细胞增殖迁移的促进作用 Note： A. Validation of RBM14 overexpression efficiency in shUSP38 cells by Western blotting. B. qPCR analysis of the effect of USP38 knockdown on RBM14 mRNA expression in gastric cancer cells. C. CCK-8 assay showing the rescue effect of RBM14 overexpression on the inhibition of cell proliferation induced by USP38 knockdown. D. Colony formation assay assessing the rescue effect of RBM14 overexpression on the clonogenic capacity of shUSP38 cells. E. EdU assay showing that RBM14 overexpression rescues the proliferation defect caused by USP38 knockdown. F. Wound healing assay assessing the rescue effect of RBM14 overexpression on cell migration. Data are presented as mean±SD. Comparisons were performed between the shUSP38+OE-RBM14 group and the shNC+OE-RBM14 group or the shUSP38 group.

Fig 7 Promotive effect of USP38 on the proliferation and migration of gastric cancer cells via stabilizing RBM14 protein