上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (4): 381-.

• 论著（基础研究） • 上一篇    下一篇

不同品系大鼠肝脏卵圆细胞增殖模型的比较研究

赵丽娟, 余 娇, 李 影, 陈 奕, 夏芳珍, 王宁荐, 陆颖理   

  1. 上海交通大学 医学院第九人民医院内分泌科, 上海 200011
  • 出版日期:2010-04-25 发布日期:2010-04-26
  • 通讯作者: 陆颖理, 电子信箱: luy662003@yahoo.com.cn。
  • 作者简介:赵丽娟（1983—）, 女, 硕士生；电子信箱: zhaolijuan83@yahoo.com.cn。
  • 基金资助:

    上海市教委基金（07ZZ35）

Comparative study of hepatic oval cell proliferation models from different rat strains

ZHAO Li-juan, YU Jiao, LI Ying, CHEN Yi, XIA Fang-zhen, WANG Ning-jian, LU Ying-li   

  1. Department of Endocrinology, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China
  • Online:2010-04-25 Published:2010-04-26
  • Supported by:

    Shanghai Education Committee Foundation, 07ZZ35

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摘要：

目的 对不同品系大鼠肝卵圆细胞增殖模型进行比较，筛选建模效率较高的大鼠品系。方法 选择F344、Wistar和SD大鼠各10只，经2-乙酰氨基芴灌胃和四氯化碳腹腔注射建立肝脏卵圆细胞增殖模型，组织学和免疫组织化学检测鉴定。荧光激活细胞筛选法分选并纯化各品系大鼠肝脏卵圆细胞，分析Thy-1.1+细胞百分率，观察Thy-1.1+细胞培养结果。结果 成功建立三种品系大鼠肝脏卵圆细胞增殖模型。流式细胞仪分选结果显示，F344、Wistar和SD大鼠Thy-1.1+细胞百分率分别为（9.46±0.99）%、（2.46±0.37）%和 (1.46±0.12)%；各品系大鼠间Thy-1.1+细胞百分率比较，差异有统计学意义（P<0.05）。细胞培养观察发现，与Wistar和SD大鼠比较，F344大鼠分选得到的肝脏卵圆细胞生长状态好且纯度较高。结论 在用于建立肝脏卵圆细胞增殖模型最为常见的三种品系大鼠中，F344大鼠所建模型的肝脏卵圆细胞活化效率最高。

关键词: 大鼠品系, 肝脏卵圆细胞, 细胞增殖模型, 细胞分选, 细胞培养

Abstract:

Objective To select the rat strain relatively more suitable for model establishment by comparison of hepatic oval cell proliferation models from different rat strains. Methods Ten F344 rats, 10 Wistar rats and 10 SD rats were selected, hepatic oval cell proliferation models were established respectively by intragastric administration of 2-acetylaminofluorene and intraperitoneally injection of carbon tetrachloride, and were identified by histological examination and immunohistochemical examination. Fluorescence-activated cell sorting was employed to separate and purify the hepatic oval cells from different rat strains, percentages of Thy-1.1+ cells were analysed, and culture results of Thy-1.1+ cells were observed. Results Hepatic oval cell proliferation models from three rat strains were successfully established. Fluorescence-activated cell sorting revealed that the percentages of Thy-1.1+ cells in F344 rats, Wistar Rats and SD rats were (9.46±0.99)%, (2.46±0.37)% and (1.46±0.12)%, respectively, and there were significant differences in percentages of Thy-1.1+cells among different rat strains (P<0.05). Observation of cell culture indicated that compared with Wistar rats and SD rats, hepatic oval cells separated from F344 rats were in better growth state and higher purifity. Conclusion Among the three rat strains most commonly used for hepatic oval cell proliferation models, hepatic oval cells from F344 rats have the highest activation efficiency.

Key words: rat strains, hepatic oval cells, animal proliferation model, cell sorting, cell culture