• Original article (Clinical research) • Previous Articles     Next Articles

Correlation between microRNA-24 expression and prognosis of aflatoxin B1-related hepatocellular carcinoma prognosis

LONG Man-mei1,2, HUANG Xiao-ying2, YAO Jin-guang2, LIU Yi-xiao1,2, MA Yun1, XIA Qiang3, LONG Xi-dai2,3   

  1. 1.Department of Pathology, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China; 2.Department of Liver Surgery, the Affiliated Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3.Department of Pathology, Youjiang Medical College for Nationalities, Baise 533000, China
  • Online:2014-12-28 Published:2014-12-30
  • Supported by:

    National Natural Science Foundation of China, 81160255, 81372639, 81460423; Guangxi Natural Science Foundation, 2013GXNSFAA019251, 2014GXNSFDA118021, 2014GXNSFAA118144; “Shu Guang” Project of Shanghai, 13SG19; Innovation Program of Shanghai Municipal Education Commission, 13YZ035

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Abstract:

Objective To explore the effects of microRNA-24 (miR-24) expression on the prognosis of aflatoxin B1 (AFB1)-related hepatocellular carcinoma (HCC). Methods A total of 207 patients with Ⅰ-Ⅱ TNM-stage HCC from high AFB1 exposure areas were selected and the corresponding surgical resection samples were collected. Expression levels of MiR-24 in the tumor tissue samples were detected by the TaqMAN-PCR technique. Cox’s regression model and logistic regression model were adopted to analyze the relationship between the miR-24 expression and the prognosis and clinicopathological features of HCC. Results Elevated expression level of miR-24 affected overall survival and tumor recurrence-free survival (P<0.01). The overall death risk and tumor recurrence risk were increased by 2.58 times and 3.75 times. This risk effect was more noticeable under high AFB1 exposure condition and the corresponding risk values were 8.13 (95%CI: 4.46-14.84) and 11.75 (95%CI: 5.15-26.79), respectively. The expression level of miR-24 was closely correlated with primary tumor size, tumor differentiation, and micro-vessel density (P<0.05), and could regulate the level of AFB1-DNA adducts. Conclusion The miR-24 expression affects the prognosis of AFB1-related HCC and regulates the clinicopathological features of HCC.

Key words: microRNA-24, hepatocellular carcinoma, aflatoxin B1, prognosis