上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (5): 540-.

• 论著（基础研究） • 上一篇    下一篇

羟基红花黄色素对离体心脏功能的影响

聂培鹤, 张文慧, 金 珠, 张 琳, 沈国舜, 张镜年, 支建明   

  1. 上海交通大学 基础医学院生理学教研室, 上海 200025
  • 出版日期:2010-05-25 发布日期:2010-05-28
  • 通讯作者: 支建明, 电子信箱: jyzhijm@shsmu.edu.cn。
  • 作者简介:聂培鹤（1982—）, 男, 硕士生；电子信箱: niepeihe@163.com。

Effects of hydroxy safflor yellow A on cardiac function of isolated hearts

NIE Pei-he, ZHANG Wen-hui, JIN Zhu, ZHANG Lin, SHEN Guo-shun, ZHANG Jing-nian, ZHI Jian-ming   

  1. Department of Physiology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-05-25 Published:2010-05-28

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摘要：

目的 研究羟基红花黄色素A（HSYA）对离体大鼠左心室收缩功能的影响。方法 42只大鼠根据实验需要随机分为正常对照组、HSYA组（1~100 μmol/L）、HSYA（30 μmol/L）对照组和HSYA（30 μmol/L）+ K+通道阻断剂组（分别为KATP阻断剂Gli、BKCa阻断剂Tea、KV阻断剂4AP和KAch阻断剂Art），每组6只。经Langendorff 离体心脏恒压灌流，依次梯度给予含有HSYA的灌流液。通过左心室内的乳胶水囊经换能器连于PowerLab生物信号采集分析系统，观察给药前后以及不同浓度HSYA对左心室收缩功能的影响，并观察不同的K+通道阻断剂对HSYA作用的影响。结果 HSYA能浓度依赖性抑制左心室收缩压、舒张末压、收缩最大速率和心率（P<0.05或P<0.01）；对舒张最大速率影响不大（P>0.05）。Gli及Tea能剂量依赖性阻断HSYA的效应（P<0.05或P<0.01），4-AP及Art对HSYA的效应没有影响（P>0.05）。结论 HSYA可呈剂量依赖性抑制心脏的收缩功能和心率，其作用机制与开放KATP和BKCa 通道有关。

关键词: 羟基红花黄色素A, 离体心脏, 心功能, K+通道

Abstract:

Objective To investigate the effects of hydroxy safflor yellow A (HSYA) on contractile function of left ventricle of isolated rat heart. Methods Forty-two rats were randomly divided into control group, HSYA (1-100 μmol/L) group, HSYA (30 μmol/L) control group, HSYA (30 μmol/L)＋glibenclamide (Gli) group, HSYA (30 μmol/L)＋tetraethylammonium (Tea) group, HSYA (30 μmol/L)＋4-aminopyridine (4-AP) group and HSYA (30 μmol/L)＋atropine (Art) group, with 6 rats in each group. Langendorff perfused heart preparation was made, and the isolated hearts were perfused with HSYA in a stepwise fashion. The effects on contractile function of left ventricle before and after treatment with different concentrations of HSYA were observed by PowerLab system from a fluid filled latex balloon in the left ventricle. The influence of different types of K+ channel blockers on the effects of HSYA was also observed. Results HSYA reduced left ventricular systolic pressure, left ventricular end-diastolic pressure, maximum rate of increase of left ventricular pressure  and heart rate in a dose-dependent manner （P<0.05 or P<0.01）, while had no significant effect on maximum rate of decrease of left ventricular pressure (P>0.05). Gli and Tea blocked the effects of HSYA in a concentration-dependent manner (P<0.05 or P<0.01), but 4-AP and Art did not block the effects of HSYA (P>0.05). Conclusion HSYA could significantly decrease the myocardial contractility and heart rate in a dose-dependent manner, and its action mechanism might be related to the opening of ATP sensitivity K+ channel and Ca2+ dependent K+ channel.

Key words: hydroxy safflor yellow A, isolated hearts, cardiac function, K+ channel