Objective To study safety and feasibility of transumbilical single-port laparoscopic cholecystectomy (LC). Methods Eighty patients undergoing LC were randomly divided into single-port group and three-port group (n=40). Operation transfer rate, operation time, blood loss, postoperative ambulation, and postoperative hospitalizing stay were compared between two groups. Incisional pain was measured by visual analogue scale. Results Compared to three-port group, there were no significant differences among the blood loss, incisional pain, postoperative ambulation, prandial time, and postoperative hospitalizing stay between the two groups. The operation time in single-port group [（62±15.5） min] was remarkably longer than that of three-port group [（34±7.8） min](P<0.001). The patients in

the single-port group were satisfactory for invisible abdominal scar. Conclusion Transumbilical single-port LC is a safe and feasible operation with satisfactory invisible abdominal scar.