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WANG Xin-lin, GENG Zhi
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Abstract:
Objective To study the expressions of P53 up-regulated modulator of apoptosis (Puma) and cytochrome C (CytC) in hippocampus tissues of rats in pilocarpineinduced status epilepticus (SE) rat model. Methods Adult male Sprague Dawley (SD) rats were intraperitoneally injected with pilocarpine of 10% (300 mg/kg) and the seizure was ceased 60 min after SE was induced. Hippocampus tissues of the rats were removed after 24 h. Brain tissues were stained by H-E and the number of neurons in CA1 area of hippocampus was observed. Then brain tissues were stained by TUNEL and the injury of hippocampus neurons was observed. The Western blotting was adopted to detect the expression of Puma in hippocampus tissues and immunohistochemical method was used to detect the CytC level in hippocampus tissues. The correlation of expressions of Puma and CytC was analyzed by the Pearson correlation analysis. Results The number of stained neurons in the CA1 area of hippocampus greatly decreased 24 h after SE and the number of TUNEL positive neurons significantly increased. Expressions of Puma and many CytC positive neurons appeared. The expression of Puma was correlated with the expression of CytC (r=0.906, P=0.000). Conclusion Apoptosis is found in hippocampus neurons of rats 24 h after SE. Expressions of Puma and CytC increase in hippocampus neurons of rats with SE and the expression of Puma is positively correlated with the expression of CytC.
Key words: status epilepticus, P53 up-regulated modulator of apoptosis, cytochrome C, rats
WANG Xin-lin, GENG Zhi. Changes of expressions of Puma and CytC in hippocampus tissues of rats with pilocarpine-induced status epilepticus[J]. , doi: 11.3969/j.issn.1674-8115.2015.02.009.
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URL: https://xuebao.shsmu.edu.cn/EN/11.3969/j.issn.1674-8115.2015.02.009
https://xuebao.shsmu.edu.cn/EN/Y2015/V35/I2/193