• Original article (Basic research) • Previous Articles     Next Articles

Application of multi-channel in vivo recording techniques to amygdala-kindling epilepsy mice

LI Jia-jia1, LI Yong-hua2, GONG Hai-qing2, LIANG Pei-ji2 , ZHANG Pu-ming2, LU Qin-chi1   

  1. 1.Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China； 2.School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    Key Basic Research Project of Shanghai Science and Technology Commission (13DJ1400303)；Natural Science Foundation of Shanghai (12ZR1413800)；Shanghai Jiao Tong University Fund for Interdisciplinary Research for Medical Applications (YG2012ZD08)；Seed Fund of Renji Hospital，Shanghai Jiao Tong University School of Medicine (RJZZ13-005)

PDF (PC)

1187

Abstract:

Objective  To provide a new experimental method for studying the mechanisms of epileptic network by integrating the multi-channel in vivo recording techniques and the establishment of epilepsy model. Methods  Multi-channel in vivo recording techniques were adopted to simultaneously record neural activities of the basolateral amygdala, cornu ammonis 1 of hippocampus, primary somatosensory cortex, and mediodorsal thalamus of amygdala-kindling mice. Meanwhile, behaviors of mice were also observed and recorded. Results  The afterdischarge was recorded in four recording regions for epilepsy from grade 1 to 5 and the duration of afterdischarge increased with the grade of epilepsy. The incidence and development of epilepsy accompanied dynamic changes of frequency-specific electrical signals. Conclusion  This experimental method may be a valuable method for study the network mechanism of the ictogenesis and epileptogenesis.

Key words: multi-channel in vivo recording techniques, temporal lobe epilepsy, amygdala-kindling mice