• Original article (Basic research) • Previous Articles     Next Articles

Preparation and preliminary application of loading PTX nanoparticles

MA Jing1, 2, SHEN Ming3, DUAN You-rong3, DU Lian-fang2   

  1. 1. Department of Ultrasound, Shanghai Songjiang Central Hospital, Shanghai General Hospital Songjiang Branch, Shanghai Jiao Tong University,Shanghai 201605, China;2.Department of Ultrasound, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China; 3. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200023, China
  • Online:2017-02-28 Published:2017-02-28
  • Supported by:

    National Natural Science Foundation of China, 81271596, 81171352, 81501483


Objective · To prepare the nanoparticles encapsulating PTX with organic biodegradable poly (lactic-co-glycolic acid)-monomethoxypoly (ethylene glycol) (PLGA-mPEG) as skeleton, and then to verify its ultrasound contrast enhanced imaging in superficial implantation tumor of pancreatic cancer and its antitumor effect in vitro. Methods · PTX-PLGA-mPEG (PTX-NPs) were prepared by double emulsion method. The preparation conditions were optimized through the design of four factors three levels orthogonal test with entrapment efficiency and loading drug efficiency as examining indexes in order to obtain optimum process parameters. Average particle size and electrical potential of the prepared PTX-NPs were examined by nano particle size and zeta potential analyzer. Size, morphology, distribution, and reunion were detected by transmission electron microscope (TEM). Ultrasound contrast enhanced imaging of PTX-NPs was observed in vitro and in superficial implantation tumor of pancreatic cancer in nude mice. Antitumor effect in vitro of PTX-NPs was detected by CCK-8 test. Results · PTX-NPs were prepared successfully. Entrapment efficiency and loading drug efficiency were (91.32±3.25)% and (2.666±0.092)%, respectively. The average size was (80.6±3.8) nm and the potential was (-13.4±1.5) mv. The morphology of PTX-NPs under TEM was spherical with excellent dispersion and no aggregation. The particles also showed a good image in vitro and in vivo. The result of CCK-8 test demonstrated that PTX-NPs significantly reduced survival rate of pancreatic carcinoma cells, and ultrasound (US) or targeted ultrasound microbubble (UTMD) given at the same time could further decrease the cell survival rate. Conclusion · PTX-NPs are good at ultrasound contrast enhanced imaging in superficial implantation tumor of pancreatic cancer. In vitro PTX-NPs have certain anti-tumor effect, which can be enhanced by US and UTMD.

Key words: PTX, nanoparticles, ultrasound imaging, pancreatic carcinoma, antitumor treatment