›› 2019, Vol. 39 ›› Issue (9): 947-.doi: 10.3969/j.issn.1674-8115.2019.09.003

• Original article (Basic research) • Previous Articles     Next Articles

Fabrication of an antibacterial hydrogel laden with adhesive liposomes for bone repairment

XIANG Yi1, 2, LIU Li-li1, 2, CUI Wen-guo1, 2   

  1. 1. Institute of Orthopaedics, Soochow University, Suzhou 215000, China; 2. Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Disease, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University of Medicine, Shanghai 200025, China
  • Online:2019-09-28 Published:2019-11-02
  • Supported by:
    National Natural Science Foundation of China, 51873107; Shanghai Talent Development Fund, 2018099; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20171906; Shanghai Jiao Tong University “Medical and Research”Program, ZH2018ZDA04）。

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Abstract: Objective · To construct an antibacterial hydrogel laden with adhesive liposomes (A-LIP-PEG) for bone repair. Methods · Adhesive liposomes (A-LIP) were fabricated based on the Schiff-based reaction between dopamine and the liposome. To prepare the liposome composite hydrogel, the A-LIPs were mixed with thiolated polyethylene glycol (4SH-PEG) which was then crosslinked with Ag+. Bone morphogenetic protein 2 (BMP-2) was loaded in the A-LIPs for its uniform dispersion and sustained release in the hydrogel network. The size distribution and zeta-potential of the A-LIPs were characterized with a particle size analyzer. The morphology of the A-LIPs was observed under transmission electron microscope. Flushing test was employed to examine the viscidity of the A-LIPs, and CCK-8 assay was conducted to demonstrate the biocompatibility of the A-LIP-PEG. Osteogenic activity of the A-LIP-PEG was evaluatedalkaline phosphatase assay and alizarin red S staining in bone marrow mesenchymal stem cells (BMSCs). Results · Strong adhesion toward soft tissue of the A-LIPs was indicatedin vitro study. A-LIP-PEG showed significant inhibition on Staphylococcus aureus. A-LIP-PEG showed superior promotion of osteogenic differentiation to hydrogel laden with liposomes (LIP-PEG), without disrupting cell proliferation. Conclusion · The A-LIP-PEG developed in this study shows nopotential to expand the application of hydrogels to drug delivery, antibacteria and bone repair.

Key words: adhesive liposome, antibacteria, hydrogels, bone repair

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